Motility of liquid stored ram spermatozoa is altered by dilution rate independent of seminal plasma concentration.
Identifieur interne : 002B21 ( Main/Exploration ); précédent : 002B20; suivant : 002B22Motility of liquid stored ram spermatozoa is altered by dilution rate independent of seminal plasma concentration.
Auteurs : M. Mata-Campuzano [Espagne] ; C. Soleilhavoup [France] ; G. Tsikis [France] ; F. Martinez-Pastor [Espagne] ; S P De Graaf [Australie] ; X. Druart [France]Source :
- Animal reproduction science [ 1873-2232 ] ; 2015.
Descripteurs français
- KwdFr :
- MESH :
- médecine vétérinaire : Conservation de semence.
- physiologie : Spermatozoïdes, Sperme.
- Animaux, Conservation de semence, Motilité des spermatozoïdes, Mâle, Ovis.
English descriptors
- KwdEn :
- MESH :
- methods : Semen Preservation.
- physiology : Semen, Spermatozoa.
- veterinary : Semen Preservation.
- Animals, Male, Sheep, Sperm Motility.
Abstract
The fertility after use of liquid stored ram semen following cervical AI rapidly decreases if semen is stored beyond 12h. The dilution of seminal plasma is often cited as a key contributor to the diminished motility and fertility of ram spermatozoa subjected to liquid preservation. Two experiments were conducted to assess the effect of spermatozoa concentration (i.e. dilution rate) and percentage of seminal plasma on the motility and viability of liquid stored ram spermatozoa. In Experiment 1, semen was diluted to one of seven concentrations ranging from 0.2 to 1.4×10(9)spermatozoa/ml with milk and assessed for motility after 3 or 24h of storage at 15°C. In Experiment 2, semen was collected and washed to remove seminal plasma before re-dilution to 0.2-1.4×10(9)spermatozoa/ml with milk containing 0%, 20% or 40% (final v/v ratio) seminal plasma and assessed for viability and motility after 3 or 24h of storage at 15°C. Whereas motility was not affected by spermatozoa concentration after 3h of storage, the proportion of progressive spermatozoa decreased after 24h of storage when spermatozoa concentration was greater than 1.0×10(9)spermatozoa/ml. The duration of preservation and the spermatozoa concentration affected spermatozoa motility but had no impact on spermatozoa viability. This negative effect of greater spermatozoa concentrations on motility was independent of the presence and the concentration of seminal plasma. The seminal plasma at both concentrations (20% and 40%) had a protective effect on spermatozoa motility after 24h of storage. These findings have the potential to improve the efficiency of cervical AI with liquid stored ram semen.
DOI: 10.1016/j.anireprosci.2015.09.004
PubMed: 26421370
Affiliations:
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Mata-Campuzano</name><affiliation wicri:level="1"><nlm:affiliation>INDEGSAL, University of León, 24071 León, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>INDEGSAL, University of León, 24071 León</wicri:regionArea><wicri:noRegion>24071 León</wicri:noRegion></affiliation></author><author><name sortKey="Soleilhavoup, C" sort="Soleilhavoup, C" uniqKey="Soleilhavoup C" first="C" last="Soleilhavoup">C. Soleilhavoup</name><affiliation wicri:level="1"><nlm:affiliation>INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; CNRS, UMR 7247, F-37380 Nouzilly, France; Université François Rabelais de Tours, F-37000 Tours, France; IFCE, F-37380 Nouzilly, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; CNRS, UMR 7247, F-37380 Nouzilly, France; Université François Rabelais de Tours, F-37000 Tours, France; IFCE, F-37380 Nouzilly</wicri:regionArea><wicri:noRegion>37380 Nouzilly</wicri:noRegion><wicri:noRegion>37380 Nouzilly</wicri:noRegion></affiliation></author><author><name sortKey="Tsikis, G" sort="Tsikis, G" uniqKey="Tsikis G" first="G" last="Tsikis">G. 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Martinez-Pastor</name><affiliation wicri:level="1"><nlm:affiliation>INDEGSAL, University of León, 24071 León, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>INDEGSAL, University of León, 24071 León</wicri:regionArea><wicri:noRegion>24071 León</wicri:noRegion></affiliation></author><author><name sortKey="De Graaf, S P" sort="De Graaf, S P" uniqKey="De Graaf S" first="S P" last="De Graaf">S P De Graaf</name><affiliation wicri:level="4"><nlm:affiliation>Faculty of Veterinary Science, The University of Sydney, NSW 2006, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Faculty of Veterinary Science, The University of Sydney, NSW 2006</wicri:regionArea><orgName type="university">Université de Sydney</orgName><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author><author><name sortKey="Druart, X" sort="Druart, X" uniqKey="Druart X" first="X" last="Druart">X. Druart</name><affiliation wicri:level="1"><nlm:affiliation>INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; CNRS, UMR 7247, F-37380 Nouzilly, France; Université François Rabelais de Tours, F-37000 Tours, France; IFCE, F-37380 Nouzilly, France. Electronic address: xavier.druart@tours.inra.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; CNRS, UMR 7247, F-37380 Nouzilly, France; Université François Rabelais de Tours, F-37000 Tours, France; IFCE, F-37380 Nouzilly</wicri:regionArea><wicri:noRegion>37380 Nouzilly</wicri:noRegion><wicri:noRegion>37380 Nouzilly</wicri:noRegion></affiliation></author></analytic><series><title level="j">Animal reproduction science</title><idno type="eISSN">1873-2232</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Male</term><term>Semen (physiology)</term><term>Semen Preservation (methods)</term><term>Semen Preservation (veterinary)</term><term>Sheep</term><term>Sperm Motility</term><term>Spermatozoa (physiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Conservation de semence ()</term><term>Conservation de semence (médecine vétérinaire)</term><term>Motilité des spermatozoïdes</term><term>Mâle</term><term>Ovis</term><term>Spermatozoïdes (physiologie)</term><term>Sperme (physiologie)</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Semen Preservation</term></keywords><keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>Conservation de semence</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Spermatozoïdes</term><term>Sperme</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Semen</term><term>Spermatozoa</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Semen Preservation</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Male</term><term>Sheep</term><term>Sperm Motility</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Conservation de semence</term><term>Motilité des spermatozoïdes</term><term>Mâle</term><term>Ovis</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The fertility after use of liquid stored ram semen following cervical AI rapidly decreases if semen is stored beyond 12h. The dilution of seminal plasma is often cited as a key contributor to the diminished motility and fertility of ram spermatozoa subjected to liquid preservation. Two experiments were conducted to assess the effect of spermatozoa concentration (i.e. dilution rate) and percentage of seminal plasma on the motility and viability of liquid stored ram spermatozoa. In Experiment 1, semen was diluted to one of seven concentrations ranging from 0.2 to 1.4×10(9)spermatozoa/ml with milk and assessed for motility after 3 or 24h of storage at 15°C. In Experiment 2, semen was collected and washed to remove seminal plasma before re-dilution to 0.2-1.4×10(9)spermatozoa/ml with milk containing 0%, 20% or 40% (final v/v ratio) seminal plasma and assessed for viability and motility after 3 or 24h of storage at 15°C. Whereas motility was not affected by spermatozoa concentration after 3h of storage, the proportion of progressive spermatozoa decreased after 24h of storage when spermatozoa concentration was greater than 1.0×10(9)spermatozoa/ml. The duration of preservation and the spermatozoa concentration affected spermatozoa motility but had no impact on spermatozoa viability. This negative effect of greater spermatozoa concentrations on motility was independent of the presence and the concentration of seminal plasma. The seminal plasma at both concentrations (20% and 40%) had a protective effect on spermatozoa motility after 24h of storage. These findings have the potential to improve the efficiency of cervical AI with liquid stored ram semen.</div></front></TEI><affiliations><list><country><li>Australie</li><li>Espagne</li><li>France</li></country><region><li>Nouvelle-Galles du Sud</li></region><settlement><li>Sydney</li></settlement><orgName><li>Université de Sydney</li></orgName></list><tree><country name="Espagne"><noRegion><name sortKey="Mata Campuzano, M" sort="Mata Campuzano, M" uniqKey="Mata Campuzano M" first="M" last="Mata-Campuzano">M. Mata-Campuzano</name></noRegion><name sortKey="Martinez Pastor, F" sort="Martinez Pastor, F" uniqKey="Martinez Pastor F" first="F" last="Martinez-Pastor">F. Martinez-Pastor</name></country><country name="France"><noRegion><name sortKey="Soleilhavoup, C" sort="Soleilhavoup, C" uniqKey="Soleilhavoup C" first="C" last="Soleilhavoup">C. Soleilhavoup</name></noRegion><name sortKey="Druart, X" sort="Druart, X" uniqKey="Druart X" first="X" last="Druart">X. Druart</name><name sortKey="Tsikis, G" sort="Tsikis, G" uniqKey="Tsikis G" first="G" last="Tsikis">G. Tsikis</name></country><country name="Australie"><region name="Nouvelle-Galles du Sud"><name sortKey="De Graaf, S P" sort="De Graaf, S P" uniqKey="De Graaf S" first="S P" last="De Graaf">S P De Graaf</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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